SUMO as a solubility tag and in vivo cleavage ...

Analyze purified protein by SDS PAGE (4-12 microliters/lane depending on expression level). Sumo protease cleavage and clean up. Change protein ...

SUMO-tagged Proteins. Small Ubiquitin-like Modifiers, also as known as SUMO proteins, are a protein family composed of various small proteins to modify function ...

SUMO proteins are small acidic proteins with distant homology to ubiquitin. At primary amino acid sequence, they are 10–20% identical to ubiquitin.

Our SUMO-tag expression system maximizes the yield of soluble, functional proteins in E. coli. SUMO functions as both a chaperonin and initiator of protein ...

In contrast to ubiquitin, SUMO is not used to tag proteins for degradation. Mature SUMO is produced when the last four amino acids of the C-terminus have ...

由 D Kuo 著作 · 2014 · 被引用 45 次 — A solution to this problem is the expression of proteins as fusions to solubility tags such as the SUMO protein. SUMO fusion proteins can be cleaved to remove ...

Sumo tag is most frequentely used as N-end fusion sequence in yeast to increase the expression and solubility of the desired recombinant protein.

由 TR Butt 著作 · 2005 · 被引用 631 次 — Fusing a tag to the target protein provides a one-step purification procedure by passing cell extracts or supernatants over an appropriate affinity matrix.

The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry and this kit allows a rapid and efficient affinity purification of active SUMO ...